FAQ

Q: What is the difference between in vivo and in vitro antibody production?

A: In vivo produced antibodies are produced in mice into ascitic fluid. In vitro produced antibodies are produced in cell culture conditions. All MedixMAB™ antibodies are produced in vitro.

Q: What species are used in the development of MedixMAB™ antibodies?

A: All MedixMAB™ antibodies are derived from mice. As a secondary antibody e.g. a rabbit anti-mouse or goat anti-mouse antibody can be used.

Q: What is hollow fiber technology?

A: Hollow fiber bioreactors are made of fibers with small pores that are placed in a shell-and-tube arrangement. The cells are placed into the extra-capillary space of the shell. Fresh medium and oxygen are supplied via intra-capillary fiber lumen. Supplements pass the fiber pores but cells and monoclonal antibodies do not. The produced monoclonal antibodies are collected from the extra-capillary medium.

Q: What is serum- or protein free cell culture medium?

A: A serum-free cell culture medium does not contain animal derived serum. A protein-free cell culture medium contains only the necessary components required for cell growth, but no proteins. MedixMAB™ antibodies are produced in both serum-free and protein-free medium.

Q: What is Protein A-affinity chromatography?

A: Protein A affinity chromatography is a protein purification technique, which uses the cell wall protein A of Staphylococcus aureus. Protein A can be used to specifically extract antibodies from cell culture medium. MedixMAB™ antibodies are purified using protein A chromatography.

Q: How is the IgG subclass determined?

A: The subclass of MedixMAB™ antibodies is determined using a commercial subclass kit.

Q: Why are MedixMAB™ antibodies provided in different buffers and not, e.g., PBS?

A: MedixMAB™ antibodies are provided in buffers, which have been selected based on stability studies. MedixMAB™ antibodies are guaranteed to remain unaltered in the product buffer at 2-8 °C until the expiry date.

Q: Can MedixMAB™ antibodies be stored in a freezer?

A: We recommend storing MedixMAB™ antibodies at 2-8 °C. Usually monoclonal antibodies tolerate at least one freeze-thaw cycle but it needs to be verified individually for each antibody.

 

Q: Is the exact epitope of MedixMAB™ antibodies known?

A: For some MedixMAB™ antibodies the epitope has been determined. If the epitope is know it is described in the product specification sheet.

Q: Do MedixMAB™ antibodies work in all applications?

A: MedixMAB™ antibodies will most probably work in sandwich and lateral flow immunoassays as these assays have been used in the development phase of the products. Other applications have not been routinely tested.

Q: If a specific/nonspecific antibody pair is used to build up an application, which one should be used as the capture antibody and which one as the detection antibody?

A: It is common to use a specific/nonspecific pair in immunoassays. If the pair is used in a traditional sandwich assay, the specific antibody should be used as the capture antibody and the nonspecific antibody as the detection antibody. In a lateral flow assay the specific antibody is usually conjugated to the particle and the nonspecific antibody is used as the capture antibody on the membrane.

Q: Is it possible to cleave MedixMAB™ antibodies into Fab fragments?

A: Using standard commercially available methods the MedixMAB™ antibodies can be cleaved into Fab fragments. Some MedixMAB™ antibodies are also available as Fab fragments.

Q: What is the production capacity of MedixMAB™ antibodies?

A: Our production and purification systems are very flexible and we can increase our production to meet the most demanding requirements. Tens or hundreds of grams are not too much.

Q: Are MedixMAB™ antibodies available in countries, which do not have a local distributor?

A: MedixMAB™ antibodies are sold worldwide. If there is no local distributor, please contact medix@medixbiochemica.com for further enquires.