MedixMDx Fast Bst RT Mix
Catalog Number: MX2107
Isothermal
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Key Documents
Product Name | MedixMDx Fast Bst RT Mix |
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Catalog Number | MX2107 |
Description | MedixMDx Fast Bst RT Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst RT Mix also contains an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx Fast Bst RT Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument. |
Application | Isothermal |
Content | 100 rxn: 1 x 1.25 ml 2x MedixMDx Fast Bst Mix; 1 x 0.2 ml RTase Amp; 1 x 0.125 ml 20x Fluorescent dye 500 rxn: 4 x 1.6 ml 2x MedixMDx Fast Bst Mix; 1 x 1 ml RTase Amp; 1 x 0.625 ml 20x Fluorescent dye |
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Storage | -20°C |
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Shipping | Cold packs / dry ice |