Molecular Reagents

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination with SYBR chemistry is required. The combination of highly selective aptamer-based fast-start formulated HiDi® DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® 2x PCR Master Mix

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination is required. The combination of highly selective aptamer-based fast-start formulated HiDi® Taq DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. The variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.

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HiDi® Taq 2x PCR Master Mix

All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!

• Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
• Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
• Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
• Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.
• Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried.

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PlexTaq® 5x qPCR Multiplex Master Mix

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes.

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RevTaq RT-PCR DNA polymerase, 50x concentrated glycerol stock

qPCR Probe 2x Lyocake Master Mix is a ready-to-use reaction mix for sensitive and reliable probe-based qPCR in all standard real-time PCR cyclers. It includes an engineered, aptamer-based fast-start DNA polymerase, optimized reaction buffer and ultrapure dNTPs. Freeze-dried qPCR Probe 2x Lyocake Master Mix can be stored at room temperature.

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qPCR Probe 2 x LyoCake Master Mix (freeze-dried)