Molecular Reagents

MedixMDx qGreen Mix is a universal intercalating dye mix for robust, sensitive, and fast qPCR. MedixMDx qGreen Mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq polymerase and intercalating dye for real-time PCR amplification. The optimized buffer chemistry and PCR enhancers and stabilizers enable rapid and sensitive qPCR.

MedixMDx qProbe Mix is a universal one-step probe mix for robust, sensitive, and fast qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase for real-time PCR amplification of single or multiplex DNA targets. MedixMDx qProbe Mix is compatible with several probes such as TaqMan® and Scorpions®.

Taq 2x PCR Master Mix is a ready-to-use reaction mix for sensitive, robust and fast PCR. The combination of aptamer-based fast-start Taq DNA polymerase, optimized reaction buffer and ulrapure dNTPs ensures consistent results, rapid set-up time and lower risk of pipetting errors. The Taq DNA polymerase has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. It provides a robust PCR performance for a wide range of PCR applications in all standard PCR cyclers.

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Taq 2x PCR Master Mix

Volcano3G® RT-PCR Probe 2x Master Mix has all necessary components for sensitive and reliable RT-qPCRs. It includes an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. With the combination of optimized reaction buffer, ultrapure dNTPs and a blue stain for visualization, Volcano3G® RT-PCR Probe 2x Master Mix reduces the need for sample extraction and sample lysis. Volcano3G® RT-PCR Probe 2x Master Mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification.

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Volcano3G® RT-PCR Probe 2x Master Mix

Volcano3G® RT-PCR Probe 2x Master Mix has all necessary components for sensitive and reliable RT-qPCRs. It includes an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. With the combination of optimized reaction buffer, ultrapure dNTPs and a blue stain for visualization, Volcano3G® RT-PCR Probe 2x Master Mix reduces the need for sample extraction and sample lysis. Volcano3G® RT-PCR Probe 2x Master Mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification. Supplied with high ROX concentration (500 nM) or low high ROX concentration (50 nM).

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Volcano3G® RT-PCR Probe 2x Master Mix (+high ROX)

Volcano3G® RT-PCR 2x Master Mix (+GreenDye) is a ready-to-use master mix for reliable qPCR and RT-qPCR in all standard PCR cyclers. The mix contains an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. The mix is supplemented with a highly sensitive GreenDye (#2000) with low polymerase inhibition. Volcano3G® RT-PCR master mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification.

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Volcano3G® RT-PCR 2x Master Mix (+GreenDye)

Volcano3G® RT-PCR 2x Master Mix (+GreenDye) is a ready-to-use master mix for reliable qPCR and RT-qPCR in all standard PCR cyclers. The mix contains an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. The mix is supplemented with a highly sensitive GreenDye (#2000) with low polymerase inhibition. Volcano3G® RT-PCR master mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification. Supplied with high ROX concentration (500 nM) or with low ROX concentration (50 nM).

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Volcano3G® RT-PCR 2x Master Mix (+GreenDye, +high ROX)

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination with SYBR chemistry is required. The combination of highly selective aptamer-based fast-start formulated HiDi® DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® 2x PCR Master Mix

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination is required. The combination of highly selective aptamer-based fast-start formulated HiDi® Taq DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. The variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.

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HiDi® Taq 2x PCR Master Mix

All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!

• Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
• Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
• Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
• Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.
• Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried.

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PlexTaq® 5x qPCR Multiplex Master Mix