Molecular Reagents

Taq hotstart DNA polymerase is an aptamer-based fast-start formulated DNA polymerase supplied with a 10x reaction buffer that has been specially designed for optimal PCR performance and DNA polymerase activity. This DNA polymerase is suitable for a wide range of PCR applications. Taq hotstart DNA polymerase has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs.

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Taq hotstart DNA polymerase

DirectBlood Genotyping PCR Kit enables sensitive, rapid and reproductible real-time PCR detection of a wide range of SNPs from EDTA blood samples without prior DNA extraction. 2x DirectBlood Genotyping PCR Mix includes an engineered, aptamer-based fast-start DNA polymerase, optimized reaction buffer and ultrapure dNTPs.

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DirectBlood Genotyping PCR Kit

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant suitable for SYBR chemistry, specially optimized for assays in which high discrimination is required. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® DNA polymerase

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant, specially optimized for assays in which high discrimination is required. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. HiDi® Taq variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® DNA Taq polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® Taq DNA polymerase

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination with SYBR chemistry is required. The combination of highly selective aptamer-based fast-start formulated HiDi® DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® 2x PCR Master Mix

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination is required. The combination of highly selective aptamer-based fast-start formulated HiDi® Taq DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. The variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.

For more information, please check :
HiDi® Taq 2x PCR Master Mix