Molecular Reagents

MedixMDx HighScript Reverse Transcriptase is a modified version of MMLV reverse transcriptase with noticeable thermostability and high enzymatic activity. This enzyme is offered as a blend with an RNase inhibitor to prevent RNA degradation. MedixMDx HighScript Reverse Transcriptase together with enhanced buffer chemistry enables fast, efficient and accurate synthesis of the cDNA molecule.

MedixMDx RNase Inhibitor is a protein that blocks the activity of a variety of ribonucleases due to very fast non-covalent binding of the inhibitor to the active sites of enzymes. Medix RNase Inhibitor is resistant to oxidative stress due to its lack of cysteine residues and it remains active at 65°C for at least 30 minutes. This RNase inhibitor blocks a wide range of RNases, but does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H.

MedixMDx qRT-Probe Mix is a universal one-step probe mix for robust, sensitive, and fast RT-qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and reverse transcriptase for efficient cDNA synthesis and real-time. The kit includes an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.

MedixMDx qRT-Probe Mix is a universal one-step probe mix for robust, sensitive, and fast RT-qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and reverse transcriptase for efficient cDNA synthesis and real-time. The kit includes a ROX additive and an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.

MedixMDx HiPlex qRT-Probe Mix is an advanced formulated one-step qRT-PCR probe mix for highly sensitive, rapid, and robust detection of RNA target templates. MedixMDx HiPlex qRT-Probe Mix uses state of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and ultra-sensitive reverse transcriptase for efficient cDNA synthesis and real-time PCR amplification. MedixMDx HiPlex qRT-Probe Mix is formulated as a 4x mix, which enables extensive multiplexing and addition of larger volumes of RNA templates to reactions. It includes an efficient thermostable reverse transcriptase with RNase inhibitor to prevent degradation of RNA templates by RNases. MedixMDx HiPlex qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.

MedixMDx qRT-PCR Lyo ready mix is a universal probe mix that allows robust, sensitive, and fast RT-qPCR for the detection of single or multiplex RNA or DNA targets. MedixMDx qRT-PCR Lyo ready mix contains optimized excipients, buffer, PCR enchancers and an antibody-regulated hot-start Taq DNA polymerase and is ready to be lyophilized to produce stable reagents at room temperature. MedixMDx qRT-PCR Lyo ready mix is ready to be lyophilized to produce stable reagents at room temperature. Upon addition of target specific primers/probes and reverse transcriptase (RTase Lyo) to the mastermix, the mixture can be lyophilized directly, without the need to add additional excipients. MedixMDx qRT-PCR Lyo ready mix is compatible with several probes such as TaqMan® and Scorpions®.

NEW: RTase dilution buffers included into the product. Easy to use, less hands-on work, less pipetting errors!

Volcano3G® RT-PCR Probe 2x Master Mix has all necessary components for sensitive and reliable RT-qPCRs. It includes an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. With the combination of optimized reaction buffer, ultrapure dNTPs and a blue stain for visualization, Volcano3G® RT-PCR Probe 2x Master Mix reduces the need for sample extraction and sample lysis. Volcano3G® RT-PCR Probe 2x Master Mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification.

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Volcano3G® RT-PCR Probe 2x Master Mix

Volcano3G® RT-PCR Probe 2x Master Mix has all necessary components for sensitive and reliable RT-qPCRs. It includes an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. With the combination of optimized reaction buffer, ultrapure dNTPs and a blue stain for visualization, Volcano3G® RT-PCR Probe 2x Master Mix reduces the need for sample extraction and sample lysis. Volcano3G® RT-PCR Probe 2x Master Mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification. Supplied with high ROX concentration (500 nM) or low high ROX concentration (50 nM).

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Volcano3G® RT-PCR Probe 2x Master Mix (+high ROX)

Volcano3G® RT-PCR 2x Master Mix (+GreenDye) is a ready-to-use master mix for reliable qPCR and RT-qPCR in all standard PCR cyclers. The mix contains an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. The mix is supplemented with a highly sensitive GreenDye (#2000) with low polymerase inhibition. Volcano3G® RT-PCR master mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification.

For more information, please check :
Volcano3G® RT-PCR 2x Master Mix (+GreenDye)

Volcano3G® RT-PCR 2x Master Mix (+GreenDye) is a ready-to-use master mix for reliable qPCR and RT-qPCR in all standard PCR cyclers. The mix contains an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. The mix is supplemented with a highly sensitive GreenDye (#2000) with low polymerase inhibition. Volcano3G® RT-PCR master mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification. Supplied with high ROX concentration (500 nM) or with low ROX concentration (50 nM).

For more information, please check :
Volcano3G® RT-PCR 2x Master Mix (+GreenDye, +high ROX)