Molecular Reagents

Volcano3G® RT-PCR 2x Master Mix (+GreenDye) is a ready-to-use master mix for reliable qPCR and RT-qPCR in all standard PCR cyclers. The mix contains an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. The mix is supplemented with a highly sensitive GreenDye (#2000) with low polymerase inhibition. Volcano3G® RT-PCR master mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification. Supplied with high ROX concentration (500 nM) or with low ROX concentration (50 nM).

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Volcano3G® RT-PCR 2x Master Mix (+GreenDye, +high ROX)

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant suitable for SYBR chemistry, specially optimized for assays in which high discrimination is required. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® DNA polymerase

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant, specially optimized for assays in which high discrimination is required. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. HiDi® Taq variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® DNA Taq polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® Taq DNA polymerase

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination with SYBR chemistry is required. The combination of highly selective aptamer-based fast-start formulated HiDi® DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® 2x PCR Master Mix

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination is required. The combination of highly selective aptamer-based fast-start formulated HiDi® Taq DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. The variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.

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HiDi® Taq 2x PCR Master Mix

5 µM, 20 µl
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2'-O-Me sensitive RT-KTQ DNA polymerase mutant

5 µM, 20 µl
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RT-KTQ2 DNA polymerase

5 µM, 20 µl
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m6A sensitive RT-KTQ DNA polymerase mutant

All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!

• Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
• Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
• Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
• Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.
• Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried.

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PlexTaq® 5x qPCR Multiplex Master Mix

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes.

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RevTaq RT-PCR DNA polymerase, 50x concentrated glycerol stock