Catalog Number: 100016

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You're reviewing:Anti-h LH 5301 SP-5
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Key Features

Liquid, may turn slightly opaque during storage

Human

CLIA, FIA

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Product Overview
Product NameAnti-h LH 5301 SP-5
Catalog Number100016
DescriptionMonoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
ApplicationCLIA, FIA
BrandMedix Biochemica
Further Specification
Form/AppearanceLiquid, may turn slightly opaque during storage
Concentration5.0 mg/ml (+/- 10 %)
Storage +2-8°C
NotesIn Pettersson et al. (1990) authors designed a rapid two-step procedure which had negligible cross-reactivity with TSH and FSH. In Pettersson et al. (1991) authors showed that some LH antibodies react differently with LH which is present in 25% of individuals. An assay utilizing 5301 was shown to react equally with LH of the two groups.
IsotypeIgG1
ClonalityMonoclonal
EpitopeB1 in a pair wise comparison as described in Pettersson et al. (1991). Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located.
Purity≥ 95 %
Affinity constantKA= 4.4 x 1011 1/M; KD= 2.3 x 10-12 M ( = 2.3 pM)
Associated ProductsNative LH antigen Lee Biosolutions 996-31
Buffer0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile6.3–7.2
Cross ReactivityLH α 13 %, LH β 170 %, FSH 5 %, hCG 138 %, TSH 0.03 %
SpecificityAntibody recognizes human luteinizing hormone (lutropin), and its beta-subunit
Storage and Shipping
Storage +2-8°C
ShippingCold packs
Shelf Life24 months
Application and References
ApplicationCLIA, FIA
ReferencesFederici, M.M., Fraser, R., Lundqvist, C., and Lankford, J.C., (1982) Production and characterization of monoclonal antibodies human lutenizing hormones. Fed. Proc., 41 Pettersson, K.S.I., and Söderholm J.R-M., (1990) Ultrasensitive two-site immunometric assay of human lutropin by time-resolved fluorometry. Clin. Chem. 36(11):1928-1933 Pettersson, K.S.I., and Söderholm J.R-M., (1991) Individual differences in lutropin immunoreactivity revealed by monoclonal antibodies. Clin. Chem. 37(3):333-340 Vilja, P., Wichmann, L., Isola, J., and Tuohimaa, P., (1988) Monoclonal-antibody based noncompetitive avidin-biotin assay for lutropin in urine.Clin. Chem. 34(8):1585-1590
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