Anti-h LH 5301 SP-5

Datasheet

Catalog Number: 100016

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Key Features

Form :

Liquid, may turn slightly opaque during storage

Species Reactivity :

Human

Application :

LF, CLIA, FIA

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Key Documents

Product Specification

Product Citations

Product Overview

Product Name	Anti-h LH 5301 SP-5
Catalog Number	100016
Description	Monoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
Application	LF, CLIA, FIA
Brand	Medix Biochemica

Further Specification

Form/Appearance	Liquid, may turn slightly opaque during storage
Concentration	5.0 mg/ml (+/- 10 %)
Notes	Nilsson et al. (2001) analyzed epitopes of 30 different LH mAbs. Antibody 5301 was classified as belonging to epitope group beta 2, recognizing intact LH, its beta subunit as well as a common variant of LH. Antibody 5301 did not cross react with TSH or FSH but did so with hCG.
Isotype	IgG1
Clonality	Monoclonal
Epitope	Beta 2 as described in Nilsson et al. (2001) Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located.
Purity	≥ 95 %
Affinity constant	KA= 4.4 x 10¹¹ 1/M; KD= 2.3 x 10^-12 M ( = 2.3 pM)
Associated Products	Native LH antigen 996-31
Buffer	0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile	6.3–7.2
Cross Reactivity	LH α 13 %, LH β 170 %, FSH 5 %, hCG 138 %, TSH 0.03 %
Specificity	Antibody recognizes human luteinizing hormone (lutropin), and its beta-subunit

Storage and Shipping

Storage	+2-8°C
Shipping	Cold packs
Shelf Life	36 months

References

References	Federici, M.M., Fraser, R., Lundqvist, C., and Lankford, J.C., (1982) Production and characterization of monoclonal antibodies human lutenizing hormones. Fed. Proc., 41 Nilsson, C., Seppälä, M., and Pettersson, K., (2001) Immunological characterization of human luteinizing hormone with special regard to a common genetic variant. J.Endocrinol. 168:10-116 Pettersson, K.S.I., and Söderholm J.R-M., (1990) Ultrasensitive two-site immunometric assay of human lutropin by time-resolved fluorometry. Clin. Chem. 36(11):1928-1933 Pettersson, K.S.I., and Söderholm J.R-M., (1991) Individual differences in lutropin immunoreactivity revealed by monoclonal antibodies. Clin. Chem. 37(3):333-340 Vilja, P., Wichmann, L., Isola, J., and Tuohimaa, P., (1988) Monoclonal-antibody based noncompetitive avidin-biotin assay for lutropin in urine.Clin. Chem. 34(8):1585-1590

Federici, M.M., Fraser, R., Lundqvist, C., and Lankford, J.C., (1982) Production and characterization of monoclonal antibodies human lutenizing hormones. Fed. Proc., 41

Nilsson, C., Seppälä, M., and Pettersson, K., (2001) Immunological characterization of human luteinizing hormone with special regard to a common genetic variant. J.Endocrinol. 168:10-116

Pettersson, K.S.I., and Söderholm J.R-M., (1990) Ultrasensitive two-site immunometric assay of human lutropin by time-resolved fluorometry. Clin. Chem. 36(11):1928-1933

Pettersson, K.S.I., and Söderholm J.R-M., (1991) Individual differences in lutropin immunoreactivity revealed by monoclonal antibodies. Clin. Chem. 37(3):333-340

Vilja, P., Wichmann, L., Isola, J., and Tuohimaa, P., (1988) Monoclonal-antibody based noncompetitive avidin-biotin assay for lutropin in urine.Clin. Chem. 34(8):1585-1590