Anti-h LH 5301 SP-5

Catalog Number: 100016

Key Features

Liquid, may turn slightly opaque during storage



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Product Citations

Product Overview
Product NameAnti-h LH 5301 SP-5
Catalog Number100016
DescriptionMonoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
ApplicationLF, CLIA, FIA
BrandMedix Biochemica
Further Specification
Form/AppearanceLiquid, may turn slightly opaque during storage
Concentration5.0 mg/ml (+/- 10 %)
NotesNilsson et al. (2001) analyzed epitopes of 30 different LH mAbs. Antibody 5301 was classified as belonging to epitope group beta 2, recognizing intact LH, its beta subunit as well as a common variant of LH. Antibody 5301 did not cross react with TSH or FSH but did so with hCG.
EpitopeBeta 2 as described in Nilsson et al. (2001) Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located.
Purity≥ 95 %
Affinity constantKA= 4.4 x 1011 1/M; KD= 2.3 x 10-12 M ( = 2.3 pM)
Associated Products

Native LH antigen 996-31

Buffer0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile6.3–7.2
Cross ReactivityLH α 13 %, LH β 170 %, FSH 5 %, hCG 138 %, TSH 0.03 %
SpecificityAntibody recognizes human luteinizing hormone (lutropin), and its beta-subunit
Storage and Shipping
Storage +2-8°C
ShippingCold packs
Shelf Life36 months

Federici, M.M., Fraser, R., Lundqvist, C., and Lankford, J.C., (1982) Production and characterization of monoclonal antibodies human lutenizing hormones. Fed. Proc., 41

Nilsson, C., Seppälä, M., and Pettersson, K., (2001) Immunological characterization of human luteinizing hormone with special regard to a common genetic variant. J.Endocrinol. 168:10-116

Pettersson, K.S.I., and Söderholm J.R-M., (1990) Ultrasensitive two-site immunometric assay of human lutropin by time-resolved fluorometry. Clin. Chem. 36(11):1928-1933

Pettersson, K.S.I., and Söderholm J.R-M., (1991) Individual differences in lutropin immunoreactivity revealed by monoclonal antibodies. Clin. Chem. 37(3):333-340

Vilja, P., Wichmann, L., Isola, J., and Tuohimaa, P., (1988) Monoclonal-antibody based noncompetitive avidin-biotin assay for lutropin in urine.Clin. Chem. 34(8):1585-1590