Anti-h LH 5301 SP-5
Catalog Number: 100016
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Key Features
Liquid, may turn slightly opaque during storage
Human
CLIA, FIA
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Key Documents
Product Citations
Product Overview
| Product Name | Anti-h LH 5301 SP-5 |
|---|---|
| Catalog Number | 100016 |
| Description | Monoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components. |
| Application | CLIA, FIA |
| Brand | Medix Biochemica |
Further Specification
| Form/Appearance | Liquid, may turn slightly opaque during storage |
|---|---|
| Concentration | 5.0 mg/ml (+/- 10 %) |
| Notes | In Pettersson et al. (1990) authors designed a rapid two-step procedure which had negligible cross-reactivity with TSH and FSH. In Pettersson et al. (1991) authors showed that some LH antibodies react differently with LH which is present in 25% of individuals. An assay utilizing 5301 was shown to react equally with LH of the two groups. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Epitope | B1 in a pair wise comparison as described in Pettersson et al. (1991). Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located. |
| Purity | ≥ 95 % |
| Affinity constant | KA= 4.4 x 1011 1/M; KD= 2.3 x 10-12 M ( = 2.3 pM) |
| Associated Products | Native LH antigen Lee Biosolutions 996-31 |
| Buffer | 0.9 % NaCI, 0.095 % NaN3 as a preservative |
| IEF Profile | 6.3–7.2 |
| Cross Reactivity | LH α 13 %, LH β 170 %, FSH 5 %, hCG 138 %, TSH 0.03 % |
| Specificity | Antibody recognizes human luteinizing hormone (lutropin), and its beta-subunit |
Storage and Shipping
| Storage | +2-8°C |
|---|---|
| Shipping | Cold packs |
| Shelf Life | 24 months |
References
| References | Federici, M.M., Fraser, R., Lundqvist, C., and Lankford, J.C., (1982) Production and characterization of monoclonal antibodies human lutenizing hormones. Fed. Proc., 41 Pettersson, K.S.I., and Söderholm J.R-M., (1990) Ultrasensitive two-site immunometric assay of human lutropin by time-resolved fluorometry. Clin. Chem. 36(11):1928-1933 Pettersson, K.S.I., and Söderholm J.R-M., (1991) Individual differences in lutropin immunoreactivity revealed by monoclonal antibodies. Clin. Chem. 37(3):333-340 Vilja, P., Wichmann, L., Isola, J., and Tuohimaa, P., (1988) Monoclonal-antibody based noncompetitive avidin-biotin assay for lutropin in urine.Clin. Chem. 34(8):1585-1590 |
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