Catalog Number: 100018

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You're reviewing:Anti-h LH 5302 SP-1
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Key Features

Liquid, may turn slightly opaque during storage

Human

CLIA, FIA

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Product Overview
Product NameAnti-h LH 5302 SP-1
Catalog Number100018
DescriptionMonoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
ApplicationCLIA, FIA
BrandMedix Biochemica
Further Specification
Form/AppearanceLiquid, may turn slightly opaque during storage
Concentration1.0 mg/ml (+/- 10 %)
NotesIn Nilsson et al. (2001) authors performed an epitope mapping analysis of 30 different monoclonal antibodies for LH. In their analysis it was shown that 5302 binds to an epitope named as beta 1. Antibodies reacting with beta 1 epitope did not react with FSH. They also showed that 5302 reacts with intact LH as well as with beta-subunit of LH while having some cross-reactivity with hCG but no cross reactivity with TSH or FSH. Clone 5302 reacted also with variant-LH.
IsotypeIgG1
ClonalityMonoclonal
EpitopeBeta 1 as described in Nilsson et al. (2001). Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located.
Purity≥ 95 %
Affinity constantKA= 4.8 x 1010 1/M; KD= 2.1 x 10-11 M ( = 21 pM)
Associated ProductsNative LH antigen Lee Biosolutions 996-31
Buffer0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile5.6–6.3
Cross ReactivityLH α 10 %, LH β 127 %, FSH 3 %, hCG 4 %, TSH 0.02 %
SpecificityAntibody recognizes human luteinizing hormone (lutropin), and its beta-subunit
Storage and Shipping
Storage +2-8°C
ShippingCold packs
Shelf Life36 months
References
ReferencesFederici, M.M., Fraser, R., Lundqvist, C., and Lankford, J.C., (1982) Production and characterization of monoclonal antibodies human lutenizing hormones. Fed. Proc., 41 Nilsson, C., Seppälä, M., and Pettersson, K., (2001) Immunological characterization of human luteinizing hormone with special regard to a common genetic variant. J.Endocrinol. 168:10-116
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