Catalog Number: 100588

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You're reviewing:Anti-h LH 5303 SPRN-5
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Key Features

Liquid, may turn slightly opaque during storage

Human

CLIA, FIA

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Product Overview
Product NameAnti-h LH 5303 SPRN-5
Catalog Number100588
DescriptionMonoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
ApplicationCLIA, FIA
BrandMedix Biochemica
Further Specification
Form/AppearanceLiquid, may turn slightly opaque during storage
Concentration5.0 mg/ml (+/- 10 %)
Storage +2-8°C
NotesIn Nilsson et al. (2001) authors performed an epitope mapping analysis of 30 different monoclonal antibodies for LH. In their analysis it was shown that 5303 binds to an epitope named as beta 1. Antibodies reacting with beta 1 epitope did not react with FSH. They also showed that 5303 reacts with intact LH as well as with the beta-subunit of LH and has no cross-reactivity with hCG, TSH or FSH. Clone 5303 reacted also with the variant-LH although with slightly lower affinity. Clone 5303 reacts also with rat LH as described in Bielmeier et al. (2004).
IsotypeIgG1
ClonalityMonoclonal
EpitopeBeta 1 as described in Nilsson et al. (2001). Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located.
Purity≥ 95 %
Affinity constantKA= 1.6 x 1011 1/M; KD= 6.3 x 10-12> M ( = 6.3 pM)
Associated ProductsNative LH antigen Lee Biosolutions 996-31
Buffer37 mM citrate, 125 mM phosphate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile6.5–7.6
Cross ReactivityLH α 9 %, LH β 10 %, FSH 1 %, hCG < 0.02 %, TSH < 0.02 %
SpecificityAntibody recognizes human luteinizing hormone (lutropin)
Storage and Shipping
Storage +2-8°C
ShippingCold packs
Shelf Life36 months
Application and References
ApplicationCLIA, FIA
ReferencesBielmeier, S.R., Best, D.S. and Narotsky, M.G. (2004) Serum hormone characterization and exogenous hormone rescue of bromodichloromethane-induced pregnancy loss in the F344 rat. Toxicol. Sci. 77:101-108 Nilsson, C., Seppälä, M., and Pettersson, K., (2001) Immunological characterization of human luteinizing hormone with special regard to a common genetic variant. J.Endocrinol. 168:10-116
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