Catalog Number: 100022

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You're reviewing:Anti-h LH 5304 SP-5
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Key Features

Liquid, may turn slightly opaque during storage

Human

CLIA, FIA

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Product Overview
Product NameAnti-h LH 5304 SP-5
Catalog Number100022
DescriptionMonoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
ApplicationCLIA, FIA
BrandMedix Biochemica
Further Specification
Form/AppearanceLiquid, may turn slightly opaque during storage
Concentration5.0 mg/ml (+/- 10 %)
NotesIn Nilsson et al. (2001) authors performed an epitope mapping analysis of 30 different monoclonal antibodies for LH. In their analysis it was shown that 5304 binds to an epitope located between groups beta 1 and 2 and the binding epitope overlaps slightly with the binding epitope of 5303. Antibodies reacting with beta 1 epitope did not react with betasubunit reacted with FSH. They also showed that 5304 reacts with intact LH as well as with beta-subunit of LH and has no cross-reactivity with hCG, TSH or FSH. Clone 5302 reacted also with variant-LH.
IsotypeIgG1
ClonalityMonoclonal
EpitopeEpitope between beta 1-2 as described in Nilsson et al. (2001). Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located.
Purity≥ 95 %
Affinity constantKA= 2.9 x 1011 1/M; KD= 3.4 x 10-12 M ( = 3.4 pM)
Associated ProductsNative LH antigen Lee Biosolutions 996-31
Buffer0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile5.6–6.3
Cross ReactivityLH 100 %, LH α 8 %, LH β 108 %, FSH 1 %, hCG <0.03 %, TSH <0.03 %
SpecificityAntibody recognizes human luteinizing hormone (lutropin), and its beta-subunit
Storage and Shipping
Storage +2-8°C
ShippingCold packs
Shelf Life36 months
References
ReferencesNilsson, C., Seppälä, M., and Pettersson, K., (2001) Immunological characterization of human luteinizing hormone with special regard to a common genetic variant. J.Endocrinol. 168:10- 116
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