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Anti-h LH 5304 SP-5

Catalog Number: 100022

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Key Features

Form :

Liquid, may turn slightly opaque during storage

Species Reactivity :

Human

Application :

CLIA, FIA

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Key Documents

Product Specification

Product Citations

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Product Overview

Product Name	Anti-h LH 5304 SP-5
Catalog Number	100022
Description	Monoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
Application	CLIA, FIA
Brand	Medix Biochemica

Further Specification

Form/Appearance	Liquid, may turn slightly opaque during storage
Concentration	5.0 mg/ml (+/- 10 %)
Notes	In Nilsson et al. (2001) authors performed an epitope mapping analysis of 30 different monoclonal antibodies for LH. In their analysis it was shown that 5304 binds to an epitope located between groups beta 1 and 2 and the binding epitope overlaps slightly with the binding epitope of 5303. Antibodies reacting with beta 1 epitope did not react with betasubunit reacted with FSH. They also showed that 5304 reacts with intact LH as well as with beta-subunit of LH and has no cross-reactivity with hCG, TSH or FSH. Clone 5302 reacted also with variant-LH.
Isotype	IgG1
Clonality	Monoclonal
Epitope	Epitope between beta 1-2 as described in Nilsson et al. (2001). Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located.
Purity	≥ 95 %
Affinity constant	KA= 2.9 x 10¹¹ 1/M; KD= 3.4 x 10^-12 M ( = 3.4 pM)
Associated Products	Native LH antigen Lee Biosolutions 996-31
Buffer	0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile	5.6–6.3
Cross Reactivity	LH 100 %, LH α 8 %, LH β 108 %, FSH 1 %, hCG <0.03 %, TSH <0.03 %
Specificity	Antibody recognizes human luteinizing hormone (lutropin), and its beta-subunit

Storage and Shipping

Storage	+2-8°C
Shipping	Cold packs
Shelf Life	36 months

References

References	Nilsson, C., Seppälä, M., and Pettersson, K., (2001) Immunological characterization of human luteinizing hormone with special regard to a common genetic variant. J.Endocrinol. 168:10- 116