Anti-h LH 5304 SP-5
Key Features
Liquid, may turn slightly opaque during storage
Human
CLIA, FIA
Custom Quote
- Bulk or bespoke? We IVDo that Request a call back
- to discuss your information requirements
Key Documents
Product Citations
Product Overview
Product Name | Anti-h LH 5304 SP-5 |
---|---|
Catalog Number | 100022 |
Description | Monoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components. |
Application | CLIA, FIA |
Brand | Medix Biochemica |
Further Specification
Form/Appearance | Liquid, may turn slightly opaque during storage |
---|---|
Concentration | 5.0 mg/ml (+/- 10 %) |
Notes | In Nilsson et al. (2001) authors performed an epitope mapping analysis of 30 different monoclonal antibodies for LH. In their analysis it was shown that 5304 binds to an epitope located between groups beta 1 and 2 and the binding epitope overlaps slightly with the binding epitope of 5303. Antibodies reacting with beta 1 epitope did not react with betasubunit reacted with FSH. They also showed that 5304 reacts with intact LH as well as with beta-subunit of LH and has no cross-reactivity with hCG, TSH or FSH. Clone 5302 reacted also with variant-LH. |
Isotype | IgG1 |
Clonality | Monoclonal |
Epitope | Epitope between beta 1-2 as described in Nilsson et al. (2001). Two antibodies binding to the same, or closely located epitopes, belong to the same group and hence cannot be used as a pair in a sandwich assay. Epitope group numbering does not give any detailed information where the epitope is located. |
Purity | ≥ 95 % |
Affinity constant | KA= 2.9 x 1011 1/M; KD= 3.4 x 10-12 M ( = 3.4 pM) |
Associated Products | Native LH antigen Lee Biosolutions 996-31 |
Buffer | 0.9 % NaCI, 0.095 % NaN3 as a preservative |
IEF Profile | 5.6–6.3 |
Cross Reactivity | LH 100 %, LH α 8 %, LH β 108 %, FSH 1 %, hCG <0.03 %, TSH <0.03 % |
Specificity | Antibody recognizes human luteinizing hormone (lutropin), and its beta-subunit |
Storage and Shipping
Storage | +2-8°C |
---|---|
Shipping | Cold packs |
Shelf Life | 36 months |
References
References | Nilsson, C., Seppälä, M., and Pettersson, K., (2001) Immunological characterization of human luteinizing hormone with special regard to a common genetic variant. J.Endocrinol. 168:10- 116 |
---|