Catalog Number: 100102

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You're reviewing:Anti-h PSA 8301 SPRN-5
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Key Features

Liquid, may turn slightly opaque during storage

Human

FIA

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Product Overview
Product NameAnti-h PSA 8301 SPRN-5
Catalog Number100102
DescriptionMonoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
ApplicationFIA
BrandMedix Biochemica
Further Specification
Form/AppearanceLiquid, may turn slightly opaque during storage
Concentration5.0 mg/ml (+/- 10 %)
Storage +2-8°C
NotesClone 8301 reacts with non-reduced and reduced intact PSA but not with reduced PSA fragments (Rye et al., 1999). In the same study affinities for free-PSA and PSA-ACT-complex were, 9.0 x 10-9 mol/l and 5.2 x 10-9, respectively. They also noted that antibodies binding to epitope group 3a form a good pair with antibodies from groups 1, 5a, 5c and 6. Clone 8301 does not cross-react with human kallikrein-2 (Black, 1999b).
IsotypeIgG1
ClonalityMonoclonal
EpitopeResidues 158–163, group 3 as described in Rye et al. (1999)
Purity≥ 95 %
Affinity constantKA= 1 x 1011 1/M
Associated ProductsNative PSA antigen, Lee Biosolutions 497-11 and 497-17
Buffer37 mM citrate, 125 mM phosphate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile6.5–7.2
Cross ReactivityPSA/α1-ACT complex 59 %, Kallikrein < 0.04 %, PAP < 0.04 %
SpecificityAntibody recognizes human prostate-specific antigen
Storage and Shipping
Storage +2-8°C
ShippingCold packs
Shelf Life36 months
Application and References
ApplicationFIA
ReferencesBlack, M.H., Grass, C.L., Leinonen, J., Stenman, U-H. and Diamandis, E.P., (1999a) Characterization of monoclonal antibodies for prostate-specific antigen and development of highly sensitive free prostate-specific antigen assays. Clin. Chem., 45(3):347-354 Black, M.H., Magklara, A., Obiezu, C.V., Melegos, D.N. and Diamandis, E.P., (1999b) Development of an ultrasensitive immuoassay for human glandular kallikrein with no crossreactivity from prostate-specific antigen. Clin. Chem., 45(6):790-799 Ferguson, R.A., Yu, H., Kalyvas, M., Zammit, S. and Diamandis, E.P., (1996) Ultrasensitive detection of prostate-specific antigen by time-resolved immunofluorometric assay and the Immulite® immunochemiluminescent third-generation assay: potential applications in prostate and breast cancer. Clin. Chem., 42(5):675-684 Magklara, A., Scorilas, A., López-Otín, C., Vizoso, F., Ruibal, A. and Diamandis, E.P., (1999) Human glandular kallikrein in breast milk, amniotic fluid, and breast cyst fluid. Clin. Chem., 45(10):1774-1780 Obiezu, C.V., Giltay, E.J., Magklara, A., Scorilas, A., Gooren, L.J.G., Yu, H., Howarth, D.J.C. and Diamandis, E.P., (2000) Serum and urinary prostate-specific antigen and urinary human glandular kallikrein concentrations are significantly increased after testosterone administration in female-to-male transsexuals. Clin. Chem., 46(6):859-862 Rye, P.D., Bormer, O.P. and Paus, E. (guest editors) (1999), ISOBM TD-3 International Workshop on monoclonal antibodies against prostate-specific antigen. Tumor Biol., 20(suppl 1):1-94
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