Anti-hCG 5014 SPTN-5
Catalog Number: 100011
Key Features
Liquid, may turn slightly opaque during storage
Mouse
Human
CLIA, FIA
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Key Documents
Product Overview
| Product Name | Anti-hCG 5014 SPTN-5 |
|---|---|
| Catalog Number | 100011 |
| Description | Monoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components. |
| Host Species | Mouse |
| Application | CLIA, FIA |
Further Specification
| Form/Appearance | Liquid, may turn slightly opaque during storage |
|---|---|
| Concentration | 5.0 mg/ml (+/- 10 %) |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Epitope | Beta-9 as described in Berger et al. (2013). The antibody recognizes both intact hCG and free ß subunit. It does not recognize hCGß core fragment. |
| Purity | ≥ 95 % |
| Affinity constant | hCG: KA= 1.6 x 109 1/M; KD= 6.1 x 10-10 M (= 0.61 nM) hCGβ: KA= 9.9 x 108 1/M; KD= 1.0 x 10-9 M (= 1.0 nM) |
| Buffer | 50 mM Na-citrate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative |
| IEF Profile | 6.4–7.5 |
| Cross Reactivity | Does not recognize hCGa, LH, FSH, or TSH |
| Specificity | Antibody recognizes human chorionic gonadotropin and its free beta subunit |
| Animal-free | Yes |
| Recommended Antigen | Native hCG antigen 189-10 and 189-11, Native ß-hCG antigen 325-11 |
Storage and Shipping
| Storage | +2–8 °C |
|---|---|
| Shipping | Cold Packs |
| Shelf Life | 36 months |
References
| References | Berger, P., Paus, E., Hemken, P.M., Sturgeon, C., Stewart, W.W., Skinner, J.P., Harwick, L.C., Saldana, S.C., Ramsay, C.S., Rupprecht, K.R., Olsen, K.H., Bidart, J.M. and Stenman, U.H. (213) Candidate epitopes for measurement of hCG and related molecules: the second ISOBM TD-7 workshop. Tumor Biol., 34: 433-457 |
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