Anti-hCG 5016 SPRN-5

Catalog Number: 100013

Key Features

Liquid, may turn slightly opaque during storage

Mouse

Human

CLIA, FIA

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Key Documents

Product Overview
Product Name Anti-hCG 5016 SPRN-5
Catalog Number 100013
Description Monoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
Host Species Mouse
Application CLIA, FIA
Further Specification
Form/Appearance Liquid, may turn slightly opaque during storage
Concentration 5.0 mg/ml (+/- 10 %)
Isotype IgG1
Clonality Monoclonal
Epitope Beta-2 as described in Berger et al. (2013). The antibody recognizes both intact hCG and free ß subunit.
Purity ≥ 95 %
Affinity constant hCG: KA= 1.6 x 109 1/M; KD= 6.4 x 10-10 M (= 0.64 nM) hCGβ: KA= 3.5 x 108 1/M; KD= 2.8 x 10-9 M (= 2.8 nM)
Buffer 37 mM citrate, 125 mM phosphate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile 6.2–6.8
Cross Reactivity Does not recognize hCGa, LH, FSH, or TSH
Specificity Antibody recognizes human chorionic gonadotropin and its free beta subunit
Animal-free Yes
Recommended Antigen

Native hCG antigens 189-10 and 189-11

Native ß-hCG antigen 325-11

Storage and Shipping
Storage +2–8 °C
Shipping Cold Packs
Shelf Life 36 months
References
References Berger, P., Paus, E., Hemken, P.M., Sturgeon, C., Stewart, W.W., Skinner, J.P., Harwick, L.C., Saldana, S.C., Ramsay, C.S., Rupprecht, K.R., Olsen, K.H., Bidart, J.M. and Stenman, U.H. (213) Candidate epitopes for measurement of hCG and related molecules: the second ISOBM TD-7 workshop. Tumor Biol., 34: 433-457