Catalog Number: 100006

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Key Features

Liquid, may turn slightly opaque during storage

Human

FIA

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Product Overview
Product NameAnti-hCG beta 5008 SP-5
Catalog Number100006
DescriptionMonoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
ApplicationFIA
BrandMedix Biochemica
Further Specification
Form/AppearanceLiquid, may turn slightly opaque during storage
Concentration5.0 mg/ml (+/- 10 %)
IsotypeIgG1
ClonalityMonoclonal
EpitopeBeta-2 as described in Berger et al. (2013). The antibody recognizes both intact hCG and free β subunit.
Purity≥ 95 %
Affinity constanthCG: KA= 2.9 x 1010 1/M; KD= 3.4 x 10-11 M (= 0.03 nM)
hCGb: KA= 4.4 x 109 1/M; KD= 2.3 x 10-10 M (= 0.23 nM)
Associated Products

Native hCG antigens, Lee Biosolutions 189-10 and 189-11
Native β-hCG antigen, Lee Biosolutions 325-11

Buffer0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile6.6 – 7.8
Cross ReactivityDoes not recognize hCGα, LH, FSH, or TSH
SpecificityAntibody recognizes human chorionic gonadotropin and its free beta subunit
Matched PairsFollowing pairs are especially recommended for free hCG beta CLIA assays: (capture) – (detection)
hCG 5012 – hCG 5004
hCG 5012 – hCG 5008
Storage and Shipping
Storage +2-8°C
ShippingCold packs
Shelf Life36 months
References
ReferencesBerger, P., Paus, E., Hemken, P.M., Sturgeon, C., Stewart, W.W., Skinner, J.P., Harwick, L.C., Saldana, S.C., Ramsay, C.S., Rupprecht, K.R., Olsen, K.H., Bidart, J.M. and Stenman, U.H. (2013) Candidate epitopes for measurement of hCG and related molecules: the second ISOBM TD-7 workshop. Tumor Biol., 34: 4033-4057
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