Catalog Number: 100368

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You're reviewing:Anti-hCG beta 5012 SPRN-5
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Key Features

Liquid, may turn slightly opaque during storage

Human

CLIA, FIA

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Product Overview
Product NameAnti-hCG beta 5012 SPRN-5
Catalog Number100368
DescriptionMonoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
ApplicationCLIA, FIA
BrandMedix Biochemica
Further Specification
Form/AppearanceLiquid, may turn slightly opaque during storage
Concentration5.0 mg/ml (+/- 10 %)
Storage +2-8°C
NotesBinding of antibody 5012 to intact hCG is sterically hindered by the α subunit. Therefore, assays developed with 5012 do not measure intact hCG.
IsotypeIgG1
ClonalityMonoclonal
EpitopeBeta-7 as described in Berger et al. (2013). The antibody recognizes free hCGβ subunit.
Purity≥ 95 %
Affinity constantKA= 1.2 x 109 1/M; KD= 8.1 x 10-10 M (= 0.81 nM)
Associated ProductsNative β-hCG antigen, Lee Biosolutions 325-11
Buffer37 mM citrate, 125 mM phosphate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative
IEF Profile6.3–7.3
Cross ReactivityDoes not recognize intact hCG, hCGα, LH, FSH, or TSH
SpecificityAntibody recognizes human chorionic gonadotropin free beta subunit
Storage and Shipping
Storage +2-8°C
ShippingCold packs
Shelf Life36 months
Application and References
ApplicationCLIA, FIA
ReferencesBerger, P., Paus, E., Hemken, P.M., Sturgeon, C., Stewart, W.W., Skinner, J.P., Harwick, L.C., Saldana, S.C., Ramsay, C.S., Rupprecht, K.R., Olsen, K.H., Bidart, J.M. and Stenman, U.H. (2013) Candidate epitopes for measurement of hCG and related molecules: the second ISOBM TD-7 workshop. Tumor Biol., 34: 4033-4057
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