Anti-hCG beta 5012 SPRN-5
Catalog Number: 100368
Key Features
Liquid, may turn slightly opaque during storage
Mouse
Human
CLIA, FIA
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Key Documents
Product Overview
| Product Name | Anti-hCG beta 5012 SPRN-5 |
|---|---|
| Catalog Number | 100368 |
| Description | Monoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components. |
| Host Species | Mouse |
| Application | CLIA, FIA |
Further Specification
| Form/Appearance | Liquid, may turn slightly opaque during storage |
|---|---|
| Concentration | 5.0 mg/ml (+/- 10 %) |
| Notes | Binding of antibody 5012 to intact hCG is sterically hindered by the α subunit. Therefore, assays developed with 5012 do not measure intact hCG. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Epitope | Beta-7 as described in Berger et al. (2013). The antibody recognizes free hCGß subunit. |
| Purity | ≥ 95 % |
| Affinity constant | KA= 1.2 x 109 1/M; KD= 8.1 x 10-10 M (= 0.81 nM) |
| Buffer | 37 mM citrate, 125 mM phosphate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative |
| IEF Profile | 6.3–7.3 |
| Cross Reactivity | Does not recognize intact hCG, hCGa, LH, FSH, or TSH |
| Specificity | Antibody recognizes human chorionic gonadotropin free beta subunit |
| Animal-free | Yes |
| Recommended Antigen | Native ß-hCG antigen 325-11 |
Storage and Shipping
| Storage | +2–8 °C |
|---|---|
| Shipping | Cold Packs |
| Shelf Life | 36 months |
References
| References | Berger, P., Paus, E., Hemken, P.M., Sturgeon, C., Stewart, W.W., Skinner, J.P., Harwick, L.C., Saldana, S.C., Ramsay, C.S., Rupprecht, K.R., Olsen, K.H., Bidart, J.M. and Stenman, U.H. (213) Candidate epitopes for measurement of hCG and related molecules: the second ISOBM TD-7 workshop. Tumor Biol., 34: 433-457 |
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