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HiDi® Taq DNA polymerase

Catalog Number: #9201

Key Features

End-Point, Real-Time

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Key Documents

Product Overview
Product Name HiDi® Taq DNA polymerase
Catalog Number #9201
Description

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant, specially optimized for assays in which high discrimination is required. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. HiDi® Taq variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® DNA Taq polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® Taq DNA polymerase

Application End-Point, Real-Time
Brand myPOLS Biotec
Further Specification
Content

S pack: 250 U, 5 U/µl, 1 x 50 µl HiDi® Taq DNA polymerase; 1 x 1.25 ml 10x HiDi reaction buffer

M pack: 250 U, 5 U/µl, 1 x 200 µl HiDi® Taq DNA polymerase; 2 x 1.25 ml 10x HiDi reaction buffer

Storage and Shipping
Storage -20°C
Shipping Cold packs
References
References

Drum, M., Kranaster, R., Ewald, C., Blasczyk, R., & Marx, A. (2014). Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele-and methylation-specific amplification. PloS one, 9(5), e96640.


Barraud, N., Létoffé, S., Beloin, C., Vinh, J., Chiappetta, G., & Ghigo, J. M. (2021). Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress. npj Biofilms and Microbiomes, 7(1), 1-11.


Miotto, O., Sekihara, M., Tachibana, S. I., Yamauchi, M., Pearson, R. D., Amato, R., ... & Mita, T. (2020). Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea. PLoS pathogens, 16(12), e1009133.


Yang, Z., Le, J. T., Hutter, D., Bradley, K. M., Overton, B. R., McLendon, C., & Benner, S. A. (2020). Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems. Biology Methods and Protocols, 5(1), bpaa004.


Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., ... & Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nature communications, 10(1), 1-13.


Madhuri, S., Bártulos, C. R., Serif, M., Lepetit, B., & Kroth, P. G. (2019). A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum. Algal Research, 39, 101469.