Molecular Reagents

qPCR Probe LyoBeads are ready-to-use, freeze-dried master mix beads with all necessary components for rapid and sensitive PCR. The combination of aptamer-based fast-start formulated DNA polymerase, optimized reaction buffer and ultrapure dNTPs in a freeze-dried format results in a cost efficient and ecological master mix that can be rehydrated within seconds in any aqueous solutions. LyoBeads can be shipped and stored at room temperature. Supplied in high profile PCR tubes (0.2 mL) or supplied in low profile PCR tubes (0.1 mL).

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qPCR Probe LyoBeads, pre-dispensed in separable 96x PCR well-plate

qPCR Probe 2x Lyocake Master Mix is a ready-to-use reaction mix for sensitive and reliable probe-based qPCR in all standard real-time PCR cyclers. It includes an engineered, aptamer-based fast-start DNA polymerase, optimized reaction buffer and ultrapure dNTPs. Freeze-dried qPCR Probe 2x Lyocake Master Mix can be stored at room temperature.

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qPCR Probe 2 x LyoCake Master Mix (freeze-dried)

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).

Comparison studies with competitor products show that the HiDi® Taq DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. 

By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.

Applications:

- Allele-specific PCR (asPCR), allele-specific amplification (ASA)

- HLA genotyping

- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)

- Mutation detection by PCR even in a high background of wild-type sequences

- Genotyping e.g., in CRISPR/Cas and TALEN approaches

This DNA polymerase is also available as a nuclease deficient variant, featuring higher robustness towards potential PCR inhibitors and compatibility with real-time dyes.

Several independently conducted studies show that HiDi® Taq DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below. 

For research use and further manufacturing. Designed and manufactured under ISO13485

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination with SYBR chemistry is required. The combination of highly selective aptamer-based fast-start formulated HiDi® DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® 2x PCR Master Mix

Isotherm3G DNA Polymerase is a mesophilic DNA polymerase, which synthesizes DNA from both DNA and RNA templates with a high strand displacement activity activity allowing simplified one-enzyme RT-LAMP reactions. It exhibits 5’ to 3’ polymerase activity but lacks any exonuclease activity. The product components have been optimized and are perfectly suited for loop-mediated isothermal amplification (LAMP) with optimal reaction temperature at 65°C.

Isotherm3G DNA Polymerase utilizes an aptamer-based warm-start feature to prevent false amplification at low temperatures. For more information, please contact us. 

The product is available also in bulk quantities with custom fillings and can be produced customized for example in a lyo-ready formulation.

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Isotherm3G DNA Polymerase 

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).

Comparison studies with competitor products show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. 

By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.

Applications:

- Allele-specific PCR (asPCR), allele-specific amplification (ASA)

- HLA genotyping

- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)

- Mutation detection by PCR even in a high background of wild-type sequences

- Genotyping e.g., in CRISPR/Cas and TALEN approaches

This polymerase is also available as a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).

Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below. 

For research use and further manufacturing. Designed and manufactured under ISO13485

Fast Bst RT Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst RT Mix also contains an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. Fast Bst RT Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Fast Bst Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activitiy, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C.