Molecular Reagents

Isotherm3G DNA Polymerase is a mesophilic DNA polymerase, which synthesizes DNA from both DNA and RNA templates with a high strand displacement activity activity allowing simplified one-enzyme RT-LAMP reactions. It exhibits 5’ to 3’ polymerase activity but lacks any exonuclease activity. The product components have been optimized and are perfectly suited for loop-mediated isothermal amplification (LAMP) with optimal reaction temperature at 65°C.

The product is available also in bulk quantities with custom fillings and can be produced customized for example in a lyo-ready formulation.

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Isotherm3G DNA Polymerase

MedixMDx Fast Taq Polymerase is a thermostable recombinant DNA polymerase expressed by Thermus aquaticus. MedixMDx Fast Taq Polymerase has 5' to 3' polymerase activity and 5' to 3' exonuclease activity, but lacks 3' to 5' exonuclease activity. The enzyme uses state-of-the-art polymerase technologies and optimized buffer chemistry for improved polymerase processivity, yield, sensitivity, and specificity. MedixMDx Fast Taq Polymerase has an error rate of approximately 1 error per 2.0 x 10^5 nucleotides incorporated.

MedixMDx HS Taq DNA Polymerase is a hot-start thermostable recombinant DNA polymerase expressed by Thermus aquaticus. MedixMDx HS Taq Polymerase has 5' to 3' polymerase activity and 5' to 3' exonuclease activity, but lacks 3' to 5' exonuclease activity. The enzyme uses state-of-the-art hot-start antibody-based technology as well as optimized buffer chemistry for high sensitivity and yield, and rapid polymerase processivity. The enzyme is ideal for complex, difficult DNA templates and is resistant to PCR inhibitors. MedixMDx HS Taq DNA Polymerase has an error rate of approximately 1 error per 2.0 x 10^5 nucleotides incorporated.

MedixMDx HS HiFi DNA Polymerase is an aptamer-based hot-start, high fidelity hyperthermophilic recombinant DNA polymerase from the archaeon Pyrococcus furiosus. MedixMDx HS HiFi DNA Polymerase exhibits 5' to 3' polymerase activity and 3' to 5' exonuclease activity. The optimized buffer chemistry facilitates high sensitivity, yield, specificity, robust and rapid polymerase processivity. The enzyme is ideal for long, complex, difficult DNA templates and is resistant to PCR inhibitors. MedixMDx HS HiFi DNA Polymerase has a lower error rate than standard Taq DNA polymerase (100x) and therefore, is suitable for PCR applications where higher accuracy is needed.

MedixMDx HighScript Reverse Transcriptase is a modified version of MMLV reverse transcriptase with noticeable thermostability and high enzymatic activity. This enzyme is offered as a blend with an RNase inhibitor to prevent RNA degradation. MedixMDx HighScript Reverse Transcriptase together with enhanced buffer chemistry enables fast, efficient and accurate synthesis of the cDNA molecule.

MedixMDx RNase Inhibitor is a protein that blocks the activity of a variety of ribonucleases due to very fast non-covalent binding of the inhibitor to the active sites of enzymes. Medix RNase Inhibitor is resistant to oxidative stress due to its lack of cysteine residues and it remains active at 65°C for at least 30 minutes. This RNase inhibitor blocks a wide range of RNases, but does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H.

MedixMDx Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activitiy, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C.

MedixMDx Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

MedixMDx qGreen Mix is a universal intercalating dye mix for robust, sensitive, and fast qPCR. MedixMDx qGreen Mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq polymerase and intercalating dye for real-time PCR amplification. The optimized buffer chemistry and PCR enhancers and stabilizers enable rapid and sensitive qPCR.

MedixMDx qProbe Mix is a universal one-step probe mix for robust, sensitive, and fast qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase for real-time PCR amplification of single or multiplex DNA targets. MedixMDx qProbe Mix is compatible with several probes such as TaqMan® and Scorpions®.