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Isotherm3G DNA Polymerase#8701
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Isotherm3G DNA Polymerase is a mesophilic DNA polymerase, which synthesizes DNA from both DNA and RNA templates with a high strand displacement activity activity allowing simplified one-enzyme RT-LAMP reactions. It exhibits 5’ to 3’ polymerase activity but lacks any exonuclease activity. The product components have been optimized and are perfectly suited for loop-mediated isothermal amplification (LAMP) with optimal reaction temperature at 65°C.
The product is available also in bulk quantities with custom fillings and can be produced customized for example in a lyo-ready formulation.
For more information, please check :
Isotherm3G DNA Polymerase -
Molecular Reagents > Clinical Chemistry
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Isothermal
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HiDi® DNA polymerase#9001
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HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).
Comparison studies with competitor products show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences.
By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.
Applications:
- Allele-specific PCR (asPCR), allele-specific amplification (ASA)
- HLA genotyping
- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)
- Mutation detection by PCR even in a high background of wild-type sequences
- Genotyping e.g., in CRISPR/Cas and TALEN approaches
This polymerase is also available as a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).
Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below.
For research use and further manufacturing. Designed and manufactured under ISO13485
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Molecular Reagents
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End-Point, Real-Time
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MedixMDx HS HiFi DNA PolymeraseMX1103
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MedixMDx HS HiFi DNA Polymerase is an aptamer-based hot-start, high fidelity hyperthermophilic recombinant DNA polymerase from the archaeon Pyrococcus furiosus. MedixMDx HS HiFi DNA Polymerase exhibits 5' to 3' polymerase activity and 3' to 5' exonuclease activity. The optimized buffer chemistry facilitates high sensitivity, yield, specificity, robust and rapid polymerase processivity. The enzyme is ideal for long, complex, difficult DNA templates and is resistant to PCR inhibitors. MedixMDx HS HiFi DNA Polymerase has a lower error rate than standard Taq DNA polymerase (100x) and therefore, is suitable for PCR applications where higher accuracy is needed.
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Molecular Reagents
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End-Point, Real-Time
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MedixMDx HighScript Reverse TranscriptaseMX1104
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MedixMDx HighScript Reverse Transcriptase is a modified version of MMLV reverse transcriptase with noticeable thermostability and high enzymatic activity. This enzyme is offered as a blend with an RNase inhibitor to prevent RNA degradation. MedixMDx HighScript Reverse Transcriptase together with enhanced buffer chemistry enables fast, efficient and accurate synthesis of the cDNA molecule.
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Molecular Reagents
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End-Point, Real-Time
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MedixMDx Fast Bst PolymeraseMX1106
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MedixMDx Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activitiy, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C.
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Molecular Reagents
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Isothermal
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MedixMDx Fast Bst Polymerase with FluorescenceMX1107
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MedixMDx Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.
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Molecular Reagents
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Isothermal
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MedixMDx qGreen Mix Separate-ROXMX2101
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MedixMDx qGreen Mix is a universal intercalating dye mix for robust, sensitive, and fast qPCR. MedixMDx qGreen Mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq polymerase and intercalating dye for real-time PCR amplification. The optimized buffer chemistry and PCR enhancers and stabilizers enable rapid and sensitive qPCR.
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Molecular Reagents
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End-Point, Real-Time
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MedixMDx qProbe Mix Separate-ROXMX2102
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MedixMDx qProbe Mix is a universal one-step probe mix for robust, sensitive, and fast qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase for real-time PCR amplification of single or multiplex DNA targets. MedixMDx qProbe Mix is compatible with several probes such as TaqMan® and Scorpions®.
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Molecular Reagents
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End-Point, Real-Time
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MedixMDx qRT-Probe Mix Separate-ROXMX2104
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MedixMDx qRT-Probe Mix is a universal one-step probe mix for robust, sensitive, and fast RT-qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and reverse transcriptase for efficient cDNA synthesis and real-time. The kit includes a ROX additive and an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.
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Molecular Reagents
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End-Point, Real-Time
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MedixMDx HiPlex qRT-Probe Mix No-ROXMX2105
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MedixMDx HiPlex qRT-Probe Mix is an advanced formulated one-step qRT-PCR probe mix for highly sensitive, rapid, and robust detection of RNA target templates. MedixMDx HiPlex qRT-Probe Mix uses state of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and ultra-sensitive reverse transcriptase for efficient cDNA synthesis and real-time PCR amplification. MedixMDx HiPlex qRT-Probe Mix is formulated as a 4x mix, which enables extensive multiplexing and addition of larger volumes of RNA templates to reactions. It includes an efficient thermostable reverse transcriptase with RNase inhibitor to prevent degradation of RNA templates by RNases. MedixMDx HiPlex qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.
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Molecular Reagents
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End-Point, Real-Time
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